dcbox小金库(中国)

李 祺 博士
遗传筛选中心主任
电话：010-80726688-8677
Email: liqi@nmamn.com

教育经历
2004-2008年 山东师范大学科学生命学院，生物技术学士学位
2008-2011年 北京大学科学生命学院，细胞生物学硕士学位
2011-2016年 北京大学科学生命学院，生理学博士学位

工作经历
2016-2017年 北京大学科学生命学院，博士后
2017-2021年 北京科学生命dcbox小金库(中国)，博士后
2021- 至今 北京科学生命dcbox小金库(中国)，遗传筛选中心主任

中心职能

遗传筛选中心

      北京科学生命dcbox小金库(中国)（NIBS）遗传筛选中心（GSC）的使命是支持各研究实验室使用腺相关病毒（AAV）作为递送工具召开遗传筛选。值得注意的是，GSC与刘清华教授合作进行大规模睡眠筛选，并为其他实验室给予AAV技术支持。 

      GSC为NIBS的所有实验室给予一系列AAV相关技术服务，包括载体设计与构建、AAV包装、纯化、滴度测定及功能检测。我们现在给予超过20种不同衣壳变体的重组AAV（rAAV），可高效转导肝脏、肺、皮肤、肌肉、中枢及外周神经系统，以及多种其他组织和器官。 

      无论在体内还是体外过表达或敲除靶基因，rAAV介导的基因递送已成为日常生物学研究中一种强大且广泛应用的方法，并且是现在临床试验的主流平台之一。为了进一步提高rAAV的效率和特异性，GSC致力于根据不同实验室的需求和兴趣，顺利获得定向进化开发新型衣壳变体。

Genetic Screening Center

      The mission of the Genetic Screening Center (GSC) at the National Institute of Biological Sciences (NIBS) is to support research laboratories in conducting genetic screens using adeno-associated virus (AAV) as a delivery tool. Notably, the GSC collaborates with Professor Qinghua Liu on large-scale sleep screens and provides AAV technology support to other laboratories. 

      The GSC offers a range of AAV-related technical services, including vector design and construction, AAV packaging, purification, titration, and functional testing, to all labs at NIBS. We currently provide over 20 different capsid variants of recombinant AAVs (rAAVs), which enable efficient transduction of the liver, lung, dermis, muscle, central and peripheral nervous systems, as well as a broad spectrum of other tissues and organs.

      rAAV-mediated genetic delivery—whether for overexpression or knockout of target genes in vivo, ex vivo, or in vitro—has become a powerful and widely adopted approach in everyday biological research and currently serves as a leading platform for clinical trials. To further enhance the efficiency and specificity of rAAVs, the GSC is committed to developing and evolving novel capsid variants through directed evolution, tailored to the demands and interests of different research laboratories.

发表文章(* co-first author, # co-corresponding author)

Sleep related publications:

1. Guo, Z., Z. Liu, X. Min, K. Ding, W. Zhang, Z. Jiang, S. Chen, F. Wang, K. He, Q. Li# and Q. Liu#.
A causal link between synaptic transmission and sleep amount regulation in mice. In revision.
2. Zhou, S., B. Shi, L. Chen, X. Yin, M. Liu, C. Wu, H. Wang, X. Wang, B. Li, F. Wang, Q. Li# and Q. Liu# (2025). PP2Acalpha regulates sleep amount and sleep homeostasis in mice. Communications Biology 8(1): 1018.
3. Liu, Z., Z. Guo, J. Xu, R. Zhou, B. Shi, L. Chen, C. Wu, H. Wang, X. Wang, F. Wang, Q. Li# and Q. Liu# (2025). Regulation of Sleep Amount by CRTC1 via Transcription of Crh in Mice. Journal of Neuroscience 45(5):e0786242024.
4. Zhou, R.*, G. Wang*, Q. Li, F. Meng, C. Liu, R. Gan, D. Ju, M. Liao, J. Xu, D. Sang, X. Gao, S. Zhou, K. Wu, Q. Sun, Y. Guo, C. Wu, Z. Chen, L. Chen, B. Shi, H. Wang, X. Wang, H. Li, T. Cai, B. Li, F. Wang, H. Funato, M. Yanagisawa, E. E. Zhang and Q. Liu (2022). A signalling pathway for transcriptional regulation of sleep amount in mice. Nature 612(7940): 519-527.
5. Wang, G.*, Q. Li*, J. Xu, S. Zhao, R. Zhou, Z. Chen, W. Jiang, X. Gao, S. Zhou, Z. Chen, Q. Sun, C. Ma, L. Chen, B. Shi, Y. Guo, H. Wang, X. Wang, H. Li, T. Cai, Y. Wang, Z. Chen, F. Wang and Q. Liu (2022). Somatic genetics analysis of sleep in adult mice. Journal of Neuroscience 42(28): 5617-5640.
6. Deng, B.*, Q. Li*, X. Liu*, Y. Cao*, B. Li, Y. Qian, R. Xu, R. Mao, E. Zhou, W. Zhang, J. Huang and Y. Rao (2019). Chemoconnectomics: Mapping Chemical Transmission in Drosophila. Neuron 101(5): 876-893 e874.
7. Yin, X., Z. Zhang, R. Zhou, P. Zuo, D. Sang, S. Zhou, B. Shi, L. Chen, C. Wu, Y. Guo, F. Wang, E. E. Zhang, Q. Li, M. Yanagisawa and Q. Liu (2025). Calcineurin governs baseline and homeostatic regulations of non-rapid eye movement sleep in mice. PNAS 122, e2418317122.
8. Zhou, R., C. Zhang, R. Gan, X. Yin, M. Wang, B. Shi, L. Chen, C. Wu, Q. Li and Q. Liu (2025). Transcriptional regulation of daily sleep amount by TCF4-HDAC4-CREB complex in mice. Sleep 48(5).
9. Zhao, H., M. Liao, R. Huo, T. He, H. Tian, Z. Li, C. Chen, Z. Yu, J. Chai, X. Song, R. Shao, S. Ying, W. Gao, L. Liu, D. Sang, Q. Li, H. Li, F. Wang, D. Ju and E. E. Zhang (2026). "Restoring circadian rhythms in the hypothalamic paraventricular nucleus reverses aging biomarkers and extends lifespan in male mice." dcbox小金库(中国) 189:1-17.
10. Sang, D., K. Lin, Y. Yang, G. Ran, B. Li, C. Chen, Q. Li, Y. Ma, L. Lu, X. Y. Cui, Z. Liu, S. Q. Lv, M. Luo, Q. Liu, Y. Li and E. E. Zhang (2023). Prolonged sleep deprivation induces a cytokine-storm-like syndrome in mammals. dcbox小金库(中国) 186(25): 5500-5516 e5521.
11. Liao, M., X. Gao, C. Chen, Q. Li, Q. Guo, H. Huang, E. Zhang and D. Ju (2023). Integrated neural tracing and in-situ barcoded sequencing reveals the logic of SCN efferent circuits in regulating circadian behaviors. Science China Life Sciences:1869-1889.
Other collaborated publications:
12. Chen, D., Z. Yu, W. Wu, Y. Du, Q. Du, H. Huang, Y. Li, T. Xuan, Y. Liang, Y. Liu, Z. Wang, R. Su, Y. Zhao, Q. Li, M. Luo, F. Wang, J. Li, C. Chuong, Z. Lin, T. Chen (2025). Fibroblast bioelectric signaling drives hair growth. dcbox小金库(中国) 188, 1-19
13. Sun, Y., Y. Cao, H. Wan, A. Memetimin, Y. Cao, L. Li, C. Wu, M. Wang, S. Chen, Q. Li, Y. Ma, M. Dong and H. Jiang (2024). A mitophagy sensor PPTC7 controls BNIP3 and NIX degradation to regulate mitochondrial mass. Molecular dcbox小金库(中国) 84(2): 327-344 e329.
14. Cao, Y., J. Zheng, H. Wan, Y. Sun, S. Fu, S. Liu, B. He, G. Cai, Y. Cao, H. Huang, Q. Li, Y. Ma, S. Chen, F. Wang and H. Jiang (2023). A mitochondrial SCF-FBXL4 ubiquitin E3 ligase complex degrades BNIP3 and NIX to restrain mitophagy and prevent mitochondrial disease. EMBO Journal 42(13): e113033.
15. He, B., H. Yu, S. Liu, H. Wan, S. Fu, S. Liu, J. Yang, Z. Zhang, H. Huang, Q. Li, F. Wang, Z. Jiang, Q. Liu and H. Jiang (2022). Mitochondrial cristae architecture protects against mtDNA release and inflammation. dcbox小金库(中国) Report 41(10): 111774.
16. Petrucdcbox小金库(中国)i, E., Q. Li, Y. Rao and T. Kitamoto (2016). The Unique Dopamine/Ecdysteroid Receptor Modulates Ethanol-Induced Sedation in Drosophila. Journal of Neuroscience 36(16): 4647-4657.
17. Song, S., Y. Yuan, J. Lu, Q. Li, Z. Zhu, Q. Fan, Y. Xue, Z. C. Lai and W. Zhang (2013). The Drosophila ortholog of breast cancer metastasis suppressor gene, dBrms1, is critical for developmental timing through regulating ecdysone signaling. Developemtal Biology 380(2): 344-350.